Skip to main content
. Author manuscript; available in PMC: 2020 Aug 22.
Published in final edited form as: Mol Cell. 2019 Jul 16;75(4):725–740.e6. doi: 10.1016/j.molcel.2019.06.011

Figure 5. Water-mediated hydrogen-bonding network of LAKE1.

Figure 5.

(A-F) Different views of water-mediated loop-loop interactions in LAKE1 involving the conserved residues of AG04 (A-C) and AG02 (D-F). Colors as in Figure 4C. Water molecules (cyan) and hydrogen bonds are depicted as spheres and dotted lines, respectively.

(G) Target cleavage assays with AG02 and mutants. See also Figures S6A–C.

(H-l) Duplex loading (H) and passenger ejection (I) of AG02 mutants that drastically reduced their target cleavage in (G). See also Figure S6F.

(J) Duplex loading of AG04 mutants. See also Figure S6G.

Black bars on panels G to J represent averages with independent replicates shown as dots.