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. Author manuscript; available in PMC: 2020 Aug 22.
Published in final edited form as: Mol Cell. 2019 Jul 16;75(4):725–740.e6. doi: 10.1016/j.molcel.2019.06.011

Figure 7. AGOs, but not PIWIs, possess LAKEs.

Figure 7.

(A-B) Interactions between loop L4 and linker MP in AG04 (A) and Siwi (B) (PDB ID: 5GUH). The van der Waals surfaces are shown as dots.

(C-D) Interactions of loops L31 and L32 in AG04 (C) and Siwi (D). The AGO-specific insert (yellow) makes space for four water molecules (LAKE2).

(E) Model of the water-mediated RISC assembly and disassembly (see the main text for details). The MD simulation predicts that mature RISC exchanges water molecules in LAKEs with the solvent (blue arrows). The water-mediated RISC disassembly is hypothetical based on our and previous studies.