Figure 4:

Differential expression of FAS and cFAS in murine tissue-specific liver and skeletal muscle FAS knockdowns.

(A) FAS protein content from liver homogenates of +/+, db/db, fasL^−/−, and fasM^−/− mice were evaluated using Western blotting. (A and B) fasL^−/− mice demonstrated significantly less FAS in the liver, while db/db mice demonstrated significantly higher FAS in the liver. *p<0.05. Error bars represent the SEM. (C) FAS protein content from anterior tibialis muscle homogenates from the same mouse groups was also evaluated using Western blotting. (C and D) fasM^−/− mice showed significantly less FAS in the muscle tissue, and db/db mice also showed significantly higher FAS in the muscle. *p<0.05. Error bars represent the SEM. (E) Whole serum was collected from all mouse groups, and cFAS was then evaluated using Western blot. fasL^−/− mice had significantly less cFAS compared to +/+ and fasM^−/−. db/db mice had significantly higher cFAS compared to all other mouse groups. **p<0.01. Error bars represent the SEM. GAPDH blot was used as loading control for all Western blots.