Table 1: Local and systemic inflammation in MSU mediated peritonitis.

Cytokine and chemokine concentrations locally (IP fluid) and systemically. WB; lysed whole blood, norm.; normalized per mg of protein in spleen. Vehicle group; vehicle+vehicle (n=4). MSU group; vehicle+MSU (n=8). MAB-mR3 group; MAB-mR3+ MSU (n=8). IL-1Ra group; IL-1Ra+MSU (n=8). Comparisons between inhibitor treated and MSU stimulated groups. Student’s t-test (two-sided) with mean ±SEM, except: G-CSF, CXCL-1 in WB and CXCL-1 in spleen; Mann-Whitney U-test with median and IQR.

IP fluid	Vehicle (n=4)	MSU stimulated (n=8)	MAB-mR3 (n=8)	IL-1Ra (n=8)
IL-6 (pg/mL)	Below detection	65.0 ±14.9	19.8 ±5.8 (p=0.014)	36.9 ±14.4 (p=0.164)
G-CSF (pg/mL)	5.0 (2.8−5.5)	77.7 (53.6−97.3)	6.6 (4.9−33.0) (p=0.015)	8.6 (7.5−17.8) (p=0.005)
CXCL-1 (pg/mL)	2.3 ±0.5	82.7 ±15.9	38.4 ±11.6 (p=0.041)	84.3. ±31.9 (p=0.965)
CCL-2 (pg/mL)	Below detection	62.5 ±11.9	26.2 ±3.6 (p=0.011)	74.2 ±14.5 (p=0.545)
CCL-3 (pg/mL)	1.2 ±0.1	4.9 ±0.8	3.6 ±0.5 (p=0.161)	5.7 ±0.9 (p=0.545)
Systemic	Vehicle (n=4)	MSU stimulated (n=8)	MAB-mR3 (n=8)	IL-1Ra (n=8)
IL-6 (pg/mL), plasma	5.0 ±3.4	69.8 ±19.4	22.7 ±5.7 (p=0.011)	29.5 ±8.5 (p=0.078)
G-CSF (pg/mL), plasma	294 ±28.4	6245 ±1055	3544 ±2743 (p=0.010)	1157 ±639 (p=0.003)
CXCL-1 (pg/mL), WB	272 (243−719)	2525 (2178−2730)	1366 (811−1889) (p=0.065)	2269 (1511−3213) (p=0.959)
MPO (ng norm.), spleen	42.5 ±7.6	145 ±9.7	89.3 ±11.4 (p=0.002)	106 ±9.7 (p=0.013)
CXCL-1 (pg norm.), spleen	16.2 (13.3−39.3)	189 (161−205)	72.5 (49.6−140) (p=0.010)	157 (95.3−238) (p=0.645)