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Demonstration of small molecule Hsp70-Bag3 screening using density drain microchip. Indexing of samples using fluorescein as internal standard (IS) (A). Percent inhibition determined for droplet samples containing potential small molecule Hsp70*-Bag3 interaction inhibitors (B). Hsp70* and Bag3 concentrations were both 1 µM and fluorescent inhibitors were assayed at 10 µM. Error bars are for n = 3 injections (from 3 droplets) for each sample.
