HuR knockout disrupts the subcellular distribution of TLR2 in the small intestinal epithelium in vivo and ex vivo. (A) Immunostaining of the small intestinal mucosa with anti-TLR2 (red), anti-E-cadherin (E-cad, green), and DAPI (blue) in mice. Scale bars, 25 μm. The experiments were repeated in the mucosal tissues obtained from three control littermates or IE-HuR−/− mice and showed similar results. (B) Immunostaining of TLR2 and E-cadherin in the intestinal organoids isolated from control littermate and IE-HuR−/− mice. In images shown in right two panels, TLR2 fluorescence was merged with E-cadherin and DIC images, respectively. Scale bars, 100 μm.