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B. subtilis and P. agglomerans cells partition into a layered coculture biofilm. Monoculture and coculture colonies grown on MSgg for 24 h were removed from the agar plate and processed for SEM. (A and B) Cells in B. subtilis monoculture biofilm (A) compared to cells of P. agglomerans in monoculture (B). (C to F) Views of outer (C), inner (D), and interface layers (E) with a schematic of a B. subtilis and P. agglomerans coculture colony (F). Bar, 1 μm.
