FIG 2.

Insertional inactivation of endA impairs the dispersion response by P. aeruginosa when exposed to glutamate or nitric oxide (NO), while insertional inactivation of eddA or eddB impairs dispersion in a manner dependent on the dispersion cue used. Biofilms were grown for 5 days in 5-fold diluted VBMM in tube reactors. Dispersion was induced by the addition of glutamate (A) or nitric oxide (NO) (B) to the growth medium. Sodium nitroprusside served as a source of NO. Absorbance of biofilm tube reactor effluents after induction of dispersion is shown. Dispersion assays were performed at least three times, with each biological replicate consisting of 4 technical replicates. Representative dispersion profiles are shown.