FIG 2.

Loss of stators impacts cellular c-di-GMP levels. (A) Representative swarm plates of the strains indicated. (B) Quantification of cellular c-di-GMP levels by LC-MS for the indicated strains grown on swarm plates. Data are expressed as picomoles of c-di-GMP per milligram (dry weight) of cells from which nucleotides were extracted. The data represent results from three independent experiments, each with three biological replicates. Values are reported as means ± SEM. Significance was determined by analysis of variance and Tukey’s post hoc test comparison for differences between strains indicated. n.s., not significant; ***, P < 0.001. As previously reported, the ΔbifA mutant is significantly different from the WT (P < 0.001).