Skip to main content
. 2019 Aug 20;10(49):5103–5117. doi: 10.18632/oncotarget.27150

Figure 4. MDA-7/IL-24 uses the clathrin-mediated endocytic (CME) pathway.

Figure 4

(A) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without Pitstop-2. After 30 min fresh growth medium was added. MTT assay was done to study the effect of MDA-7/IL-24. MDA-7/IL-24 inhibited the proliferation of cells, while in the presence of inhibitors; it failed to inhibit the proliferation of cells. (B) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without chlorpromazine (10 μg/mL) or monodansylcadaverine (200 μM). After 30 min cells received fresh growth medium. MTT assay was done to study the effect of MDA-7/IL-24. (C) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without EIPA. After 30 min fresh growth medium was added. MTT assay was done to study the effect of MDA-7/IL-24. (D) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without Genistein (200 μM) or Nystatin (20 μM). After 30 minutes fresh growth medium was added. MTT assay was done to study the effect of MDA-7/IL-24. *** P<0.001, ns: not significant. (E) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without Pitstop-2. Western blotting showing the expression of receptors, biotinylated membrane fraction was isolated and probed with the indicated antibodies. Na-K-ATPase was used as control. (F) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without chlorpromazine (10 μg/mL) or monodansylcadaverine (200 μM). After 30 min cells received fresh growth medium. Western blotting showing the expression of receptors, biotinylated membrane fraction was isolated and probed with the indicated antibodies. (G) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without Genistein (200 μM) or Nystatin (20 μM). Western blotting showing the expression of receptors, biotinylated membrane fraction was isolated and probed with the indicated antibodies. (H) DU-145 cells were treated with His-MDA-7/IL-24 (10 μg/mL) for 30 min with/without EIPA. After 30 min fresh growth medium was added. Western blotting showing the expression of receptors, biotinylated membrane fraction was isolated and probed with the indicated antibodies.