Fig. 8.

CCDC103 RNA expression levels (a) from control testicular cells (TC), culture Sertoli cells (SC), sperm (SZ), oocytes, nasal cells (nasal) and white blood cells (WBC). SYBR Green was the fluorescent dye used. Statistical significance was determined using the Mann-Whitney test, with alpha < 0.05. WBC was used as a calibrator and B2M as the reference gene. Resume of all possible combinations between samples (b). The C (calibrator) column means that this tissue was used as calibrator. For example, using WBC as a calibrator, it was observed that samples (S) from TC, SC, oocytes, and nasal cells present a reduced expression (↓), while SZ has a higher expression (↑) than WBC. ⇔ means that there were no statistically significant differences between the two groups. Controls: for TC cells are samples obtained from men with conserved spermatogenesis that are under infertility treatments; for the remaining cells, are cells from healthy individuals, without PCD/KS or other related diseases found