Table 1.
Genes of interest and associated functions of interest arising over time during human umbilical cord perivascular cell in vitro culture
| Symbol | Log2 FCa | Functionb |
|---|---|---|
| Early passages (3–5) | ||
| EPN1 | 0.3c | Endocytosis of clathrin‐coated vesicles; cell signaling 70 |
| FUZ | −0.4 | GO: embryonic organ morphogenesis (skeletal system) |
| GLI1 | −0.6 | GO: embryonic organ morphogenesis |
| HIPK2 | −0.5 | GO: embryonic organ morphogenesis, negative regulation of transcription, DNA templated |
| PCGF2 | −0.6 | GO: embryonic organ morphogenesis (skeletal system), negative regulation of transcription, DNA templated, chromosome and chromatin organization |
| SOX11 | −2.0 | GO: embryonic organ morphogenesis (skeletal system), negative regulation of transcription, DNA templated |
| Mid‐passages (6–9) | ||
| NCAM1 | −3.5 | Cell interactions during nervous, immune system development |
| NEFH | −1.8 | Maintains neuronal caliber; intracellular transport to axons, dendrites |
| SOX11 | −3.5 | Regulation of embryonic development, determination of cell fate (TF); GO: regulation of glial cell proliferation, spinal cord development |
| TMSB15A/B | −3.3c | Tissue regeneration: module migration, angiogenesis, immune responses 71, 72 |
| Presenescent passages (10–12): Decreased expression | ||
| CREB1 | −1.1 | GO: regulation of glial cell proliferation |
| GLI2 | −1.3 | GO: ventral spinal cord development, spinal cord motor neuron differentiation |
| IDH2 | −1.4 | GO: regulation of glial cell proliferation |
| LMO4 | −1.2 | GO: ventral spinal cord development, spinal cord motor neuron differentiation |
| ZC4H2 | −1.0 | GO: ventral spinal cord development, spinal cord motor neuron differentiation |
| Presenescent passages (10–12): Increased expression | ||
| COL8A2 | 3.9 | Encodes collagen in basement membrane of the corneal endothelium |
| DMD | 2.2 | GO: muscle organ development; regulation of skeletal and cardiac muscle contraction |
| DSP | 3.3 | Skin development and strength by anchoring intermediate filaments to desmosomal plaques |
| HIST1H2B‐C, ‐E, ‐F, ‐G, ‐H, ‐I, ‐K | 4.2 | Nucleosome structure, compaction of chromatin, antibacterial and antifungal antimicrobial activity |
| IDS | 1.5 | Lysosomal degradation of heparan sulfate, dermatan sulfate; chondrogenesis |
| KRT17 // JUP | 1.7 | Encodes type I intermediate filament chain keratin 17 // cytoplasmic protein of desmosomes, intermediate junctions; forms cadherin complexes |
| MFAP5 | 5.2 | Promotes attachment of cells to microfibrils of extracellular matrix |
| PCDH7 | 3.6 | GO: cell adhesion, cell–cell signaling, platelet degranulation |
| PSTPIP2 | 1.3 | GO: actin filament polymerization, cell migration, cytoskeleton organization |
| TAGLN | 1.1 | Early marker of smooth muscle differentiation, tumor suppressor; platelet degranulation |
| HIST2H2AA3/4 | 2.7 | DNA damage processes: cell cycle regulation, apoptosis |
| NSG1 | 3.0 | DNA damage processes: cell cycle regulation, apoptosis |
| RGCC | 2.7 | DNA damage processes: cell cycle regulation, apoptosis |
| SRGN | 1.3 | DNA damage processes: cell cycle regulation, apoptosis; GO: platelet degranulation, negative regulation of bone mineralization |
| THBS1 | 2.5 | DNA damage processes: cell cycle regulation, apoptosis |
| SLC20A2 | 1.4 | Solute carrier: cell metabolism, transmembrane support and signaling |
| SLC22A4 | 1.7 | Solute carrier: cell metabolism, transmembrane support and signaling |
| SLC31A2 | 1.1 | Solute carrier: cell metabolism, transmembrane support and signaling |
| WNT5A | 1.3 | Canonical WNT pathway activation 73 |
| WNT5B | 1.4 | Noncanonical WNT pathway activation 73 |
a
Average log2 fold change of most highly expressed probe set (if >1) for all designated passages; positive number is upregulated and negative is downregulated versus P2.
b
Taken from gene's RefSeq summary as provided by www.ncbi.nlm.nih.gov/gene or published literature search unless otherwise stated.
c
Not statistically significant at any of the designated passages, q > .05.
Abbreviations: //, nonspecific probe set; FC, fold change; GO, Gene Ontology biological process terms of interest; TF, transcription factor.