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. 2019 May 30;8(9):959–970. doi: 10.1002/sctm.18-0222

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© 2019 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Human placental stem cell (HPSC)‐derived conditioned medium (CM) improves cell viability and proliferation. HPSC‐CM resulted in concentration‐dependent increases of cells in proliferation for both renal (primary renal cells [PRCs]; A) and endothelial cells (human umbilical vein endothelial cell [HUVEC]; C) at 1, 2, and 3 days (analysis of variance, Tukey post hoc analysis at p < .05; (A) 1 day [*, with 0×], 2 and 3 day [*, with 0×, 0.25×, 0.5×, 1×; **, with 2×; ***, with 4×; +, with 0×, 0.25×, 0.5×]; (C) *, with 0×, 0.25×, 0.5×, 1×; **, with 2×; ***, with 4×; +, with 0×; n = 3). Viable cells (green) were prominent in the CM‐treated groups in a concentration‐dependent manner at 3 days for both PRC (B) and HUVEC (D) (0‐, 0.25‐, 0.5‐, 1‐, 2‐, and 4‐fold; ×200, Bar = 100 μm). Dead cells fluoresce red. As a positive control, cells were also cultured with respective normal GM. Abbreviation: GM, growth medium.