Fig. 3.

The effects of CKIP-1 knock-down on high-glucose treated HRECs in terms of cell viability, oxidative stress, inflammation and apoptosis. a Relative CKIP-1 mRNA levels were detected by Real-Time qPCR (n = 3). b Relative CKIP-1 protein levels were detected by Western Blot (n = 3). c CCK-8 assay kit was utilized to detect cell viability. d MDA levels, SOD activity and GSH-PX activity were detected by MDA assay kit, SOD kit abd GSH-PX kit respectively (n = 3). Inflammation associated cytokines (TNF-α, IL-6 and IL-1β) were detected by ELISA (n = 3). e Calcein-AM/PI double stain kit was employed to detect cell apoptosis (Scale bar is 200 μm). f Apoptosis associated proteins (Bcl-2, Bax and Cleaved Caspase 3) were detected by Western Blot (n = 3). The data above in one experiments were repeated at least 3 times and performed as mean ± standard deviation (SD), *P < 0.05, **P < 0.01 and ***P < 0.001