Fig. 4.

Nrf2/ARE signaling pathway was activated by CKIP-1 in high-glucose treated HRECs. a, b Co-IP was used to investigate the protein–protein interactions of CKIP-1 and Nrf2 (n = 3). c Luciferase reporter gene system was used to detect the transcriptional activity of Nrf2 (n = 3). d Western Blot was used to detect nuclear Nrf2 and cytoplasm Nrf2 expressions (n = 3). e Immunofluorescence assay was performed to detect the expression levels and cellular localization of Nrf2 (Scale bar is 50 μm). f The downstream targets of Nrf2 (including HO-1, NQO-1, γGCS and SOD) were detected by Western Blot (n = 3). The data above in one experiments were repeated at least 3 times and performed as mean ± standard deviation (SD), *P < 0.05, **P < 0.01 and ***P < 0.001