Fig. 5.

Knock-down of Nrf2 reverses the effects of overexpressed CKIP-1 on cell viability, oxidative stress, inflammation and apoptosis of high-glucose treated HRECs. a Real-Time qPCR was used to detect relative Nrf2 mRNA levels (n = 3). b Western Blot was employed to detect relative Nrf2 protein expressions (n = 3). c Cell viability was evaluated by CCK-8 assay kit (n = 3). d MDA levels, SOD activity and GSH-PX activity were detected by MDA assay kit, SOD kit abd GSH-PX kit respectively (n = 3). Inflammation associated cytokines (TNF-α, IL-6 and IL-1β) were detected by ELISA (n = 3). e Calcein-AM/PI double stain kit was employed to detect cell apoptosis (Scale bar is 200 μm). f Apoptosis associated proteins (Bcl-2, Bax and Cleaved Caspase 3) were detected by Western Blot (n = 3). The data above in one experiments were repeated at least 3 times and performed as mean ± standard deviation (SD), *P < 0.05, **P < 0.01 and ***P < 0.001