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Expression levels of female-specific protein 800 (fs800) at various developmental stages of S. japonicum as measured by qPCR. qPCR was used to analyze fs800 in life-cycle stages: eggs, cercariae, schistosomula (at 16 dpi) and adult worms at 24 and 42 dpi (F(4,10) = 173.5, DF = 4, P < 0.0001). Data represent the mean ± SEM of three independent experiments (three biological replicates), compared to the egg group. *P < 0.05, **P < 0.01 by two-way ANOVA
