Fig. 5.

Effects of Sjfs800 knockdown on pairing rate, egg production and reproductive organ development. a The male–female pairing rate of worms transfected with Sjfs800 siRNA1 was reduced by approximately 70% compared with the rate in the scrambled siRNA-treated group (F_{(2, 6)} = 660.9, P < 0.0001). b The eggs in the culture medium were collected on day 10 and counted using light microscopy. SiNCTRL represents negative control siRNA group; SiCTRL represents scrambled siRNA (F_{(2, 6)} = 36.81, P = 0.0004). c The eggs in female uterus per couple were determined using light microscopy after 10 days of cultivation (F_{(2, 6)} = 66.59, P < 0.0001). Asterisks show statistical differences (*P < 0.05, **P < 0.01) tested by one-way ANOVA, compared to the mock group. Data are expressed as the mean ± SEM of three independent experiments three biological replicates