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. 2019 Aug 23;10:268. doi: 10.1186/s13287-019-1382-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — PBX1 activated the ATK/GSK3β signaling pathway in induced pluripotent stem cells. a, b Transduced HF-MSCs were harvested on day 7 to assess the levels of phospho-AKT, phospho-GSK3β, p16, p21, and β-catenin. GAPDH and HISTONE were used as loading controls. The value in SOMK-transduced HF-MSCs as the control group was set as 1.0. c Immunofluorescence analysis of β-catenin and p53 expression and localization in transduced HF-MSCs (bar, 100 μm). d, e Transduced HF-MSCs were harvested on day 21 to assess the levels of phospho-AKT, phospho-GSK3β, p16, p21, NANOG, and β-catenin. GAPDH and HISTONE were used as endogenous controls for equal loading. The value in SOMK-transduced HF-MSCs as the control group was set as 1.0. *P < 0.05; **P < 0.01; ***P < 0.001