Mitochondrial number and enzymatic activity are increased in cells from lean and obese asthmatic subjects. (A) Mitochondrial DNA copy number (normalized to platelet number) in platelets and from healthy lean, asthmatic lean, healthy obese, and asthmatic obese individuals. (B) Mitochondrial DNA copy number normalized to corresponding nuclear DNA copy number in airway epithelial cells from the same groups. Bars in (A, B) represent mean ± SEM. (C) Citrate synthase activity (represented as a fold change of the respective healthy lean group) in airway epithelial cells (black bars) and platelets (white bars). (D) Enzymatic activity of complexes I, II, and IV measured in airway epithelial cells and represented as a fold change of the healthy lean group. n = 10 for platelets and n = 5 for airway epithelial cells. Statistical significance determined by one-way ANOVA. #p < 0.05, *p < 0.01 versus respective healthy control. Color images are available online.