FIG. 7.

NO inhibits respiration and complex IV but not glycolysis in cells from lean asthmatic subjects. (A, B) Basal and maximal respiratory rates in (A) airway epithelial cells and (B) platelets isolated from lean and obese asthmatic individuals and treated with (white bars) or without (black bars) c-PTIO (100 μM). (C) Basal ECAR in airway epithelial cells and platelets isolated from lean and obese asthmatic individuals and treated with or without c-PTIO. (D) Representative trace of platelets permeabilized with digitonin (20 μg/mL) and treated with TMPD (0.5 mM) and ascorbate (2 mM) to measure oxygen consumption by complex IV in the presence and absence of c-PTIO. (E) Quantification of eight traces similar to those shown in (D). Statistical significance determined by one-way ANOVA. n = 4; Data are mean ± SEM ^#p < 0.05 and *p < 0.01. c-PTIO, 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl 3-oxide; TMPD, tetramethyl-p-phenylenediamine. Color images are available online.