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. 2019 Aug 23;28(17):1151–1166. doi: 10.1089/scd.2019.0090

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© Ratnesh K. Singh et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — Differentiation of hESCs to retinal tissue (retinal organoids) and immunocytochemical characterization of retinal organoids before transplantation. (a) Schematic of three-dimensional retinal organoid differentiation protocol. (b–f) Representative bright-field images of retinal differentiation stages in culture. (g–n) Immunocytochemistry of hESC-derived retinal tissue (9–10 weeks) with antibodies specific to PAX6, NEUROD1, CALB2, CHX10, OTX2, ZO-1, BLIMP1, CRX, and BRN3A. Insets in panel j represent the magnification of the area marked with asterisks. hESC, human embryonic stem cell.