Fig. 3.

Binding linear density (BLD) of each experiment plotted versus most probable encounter duration (red dots) for each pMHC (A–E). Most probable (MP) encounter duration D_e was calculated as D_e = L/V_avg, where L = 35 nm is the molecular length of the bond, including intermediate antibodies, and V_avg is the peak of the microspheres’ velocity distribution. Data fits for 2 bond models are shown: The dashed lines show the fit with the classical k_on model, while the full lines show the fit with the minimal encounter duration model (t_on model).