Fig. 2.

LDs exhibit varying internal structure under different cellular states. Top row shows entire LD with Insets representing the fast Fourier transforms (FFT). Bottom rows provide a zoomed-in view at a central slice through the LDs (periphery-center). (Scale bars, 100 nm.) LDs in normally cycling cultures (control) are amorphous, and the FFT only exhibits modulations associated with EM images acquired at defocus. Mitotically arrested cells show a uniform concentric layered structure at their periphery (double-sided arrow), represented by a ring in the FFT. Starved cells also exhibit a layered structure (double-sided arrow), as well as crystalline nanodomains at the center (arrowheads), represented by bright peaks superimposed on a ring of the same spacing in the FFT. Starved cells exposed to heat shock of 43 °C lose the crystalline structure and are amorphous. LDs in Arsenite-treated cells (30′ As) are distorted and exhibit bilayer-like structure at the periphery. The FFT was generated from the framed area.