Cys139 of SCE1 is required for resistance to Pst DC3000. (A and B) Plants were inoculated with 105 cfu/mL Pst DC3000 (A) or Pst DC3000 (avrB) (B), and leaf discs were assayed for bacterial growth at 3 dpi. Data points represent mean ± SD (n = 6 biological replicates), with asterisks indicating significant difference from WT (P < 0.05, Student’s t test). (C) Plants were inoculated with 106 cfu/mL Pst DC3000, and leaf tissue was harvested at the stated times. The expression of PR1 was analyzed by qPCR and normalized against the constitutively expressed UBQ5. Data points represent mean ± SD (n = 3) of 3 independent biological samples, and asterisks represent significant differences between the indicated samples (*P < 0.05, ***P < 0.0001, Student’s t test). (D) Plants were inoculated with 106 cfu/mL Pst DC3000 (avrB), and PR1 expression was analyzed as in C.