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. 2019 Aug 7;116(34):17045–17050. doi: 10.1073/pnas.1902091116

Fig. 1.

Fig. 1.

Treg depletion delays recovery after CCI. (A) WT mice underwent CCI at week 0. Mechanical allodynia was determined using the Von Frey test over a period of 12 wk. Five and seven weeks after injury, mice were injected with control IgG (con IgG) or PC-61 (500 µg per i.p. injection, gray arrows) to deplete Tregs (n = 10 per group). **P < 0.01 con IgG vs. PC-61 ipsilateral paw, ##P < 0.01 wk 6–10 vs. week 5 PC-61 ipsilateral paw. (B–E) Percentage of splenic CD3+ T cells (B) and CD25+FoxP3+ Tregs (C) (population: CD3+ T cells) was quantified by flow cytometry 12 wpi. Shown are scatterplots from representative experiments. Values in each quadrant are the percentage of positive cells. Quantification shows the mean ± SEM. (D) Correlation of the percentage of splenic Tregs with the withdrawal threshold at 12 wpi (n = 10 per sex). (E) Quantification of the percentage of different splenic immune cell subsets of CCI animals 12 wpi (n = 10 each group). ****P < 0.0001; ns, not significant.