Fig. 6.

C_i uptake of D66 and bsti1-3. C_i uptake and C_i accumulation were measured in D66 and bsti1-3 using the silicone oil uptake method (Materials and Methods). Cells were grown in high CO₂ and then acclimated to ambient CO₂ for 12 h prior to the assays. Cells were harvested and depleted of endogenous C_i before running the assays. A time course of intracellular C_i accumulation (A) and CO₂ fixation (B) is shown for pH 8.4. Triplicate samples were run for each time point. The added H¹⁴CO₃⁻ concentration was 50 μM.