Fig. 4.
pC1 dsx neurons are cholinergic. (A) Immunostaining of GFP in adult female genotype UAS > stop > mCD8::GFP;R26E01-Gal4/dsxFLP counterstained with dsx. (B and C) Higher-magnification (60× objective) of anti-mCD8 (green) (B1 and C1), anti-dsx (red) (B2 and C2), and a merger of B1 and C1 and B2 and C2 (B3 and C3). (D) Immunostaining of cell membrane in the adult female genotype UAS > stop > mCD8::GFP;R26E01-Gal4/dsxFLP counterstained with ChAT. (E and F) Higher-magnification (60× objective) views of anti-mCD8 (green) (E1 and F1), anti-ChAT (red) (E2 and F2), and a merger of E1 and F1 and E2 and F2 (E3 and F3). (Scale bars: 50 µm in A and D; 25 µm in B, C, E, and F.)