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. 2019 Aug 1;116(34):17096–17104. doi: 10.1073/pnas.1906083116

Fig. 4.

Fig. 4.

Accumulation of melianol and salannin and expression of MaOSC1, MaCYP71CD2, and MaCYP71BQ5 in M. azedarach. (A) Estimated concentrations (mg/g DW, n = 4 ± SE) of the protolimonoid melianol (4) and the seco-C-ring limonoid salannin in extracts from M. azedarach leaf, root, and petiole tissue. (B) Normalized expression of MaOSC1, MaCYP71CD2, and MaCYP71BQ5 relative to Maß-actin in RNA from leaves, roots, and petioles of M. azedarach by qRT-PCR. Relative expression levels were calculated using the ΔΔCq method (58) (n = 4 ± SE). T-test significance values are indicated: not significant (NS), P value ≤ 0.05 (*), 0.01 (**), or ≤ 0.001 (***).