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. 2019 Aug 1;116(34):16711–16716. doi: 10.1073/pnas.1903500116

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Fig. 2. — MscL is essential for sPLA₂–M–MscL communication. (A) The release of calcein from 1:1 DOPC:DOPG vesicles can be monitored spectroscopically over time. sPLA₂ is added at 10 min, before monitoring calcein fluorescence for 100 min. Release of calcein from vesicles results in a fluorescence increase as the dye dilutes in external solution and self-quenching becomes inefficient. Error bars show propagated error of 1 SD (n = 3). (B) Total calcein flux at 100 min for vesicles ± MscL. sPLA₂-concentration-dependent calcein flux is only observed for vesicles containing MscL. Error bars show 1 SD (n = 3).