Fig. 3.

The sPLA₂–M–MscL network can be controlled through calcium chelation. (A) sPLA₂ inactivation, and hence network activation, can be achieved through the addition of increasing concentrations of the Ca²⁺ chelator EDTA. Error bars = 1 SD (n = 3). (B) Monitoring reactivation of the network using calcein flux after the addition of Ca²⁺ (0–10.0 mM) to solutions containing the network and 2.5 mM EDTA. Error bars show 1 SD (n = 3). (C) Total calcein flux after 60 min for 1:1 DOPC:DOPG vesicles ± MscL showing that the MscL channel is necessary for network reactivation with Ca²⁺. Error bars show 1 SD (n = 3).