Fig. 1.
Identification of Cenpc1 and Qrfp as putative narcolepsy gene markers. (A–C) Differential RNA sequencing analysis between Hcrtko/ko (OXKO), or Hcrt-Ataxin-3 Tg (OXAt) mice, and their respective control littermates (Ctrl), was performed in 3 brain regions (brainstem, cortex, and hypothalamus). Depicted are the differential expressions of Hcrt, Cenpc1, and Qrfp in each region. Note that Qrfp mRNA levels are about 50% reduced in the hypothalamus of Hcrt-ataxin-3 transgenic mice relative to WT littermates. Read counts has been normalized with DESeq2 package. (D–F) Expression of Hcrt, Pmch, and Qrfp in the hypothalamus of Hcrt-Ataxin-3 transgenic and WT littermates at 4 developmental time points (1, 3, 6, and 9 wk after birth). Shown are values (mean ± SEM, arbitrary units) derived from qRT-PCR analysis (t test, n = 3 mice; *P = 0.023; **P < 0.01; ***P < 0.001).