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. 2019 Aug 7;116(34):17105–17114. doi: 10.1073/pnas.1907968116

Fig. 1.

Fig. 1.

Expression of CsBRC1 in cultivated cucumbers and its wild ancestor Cucumis sativus var. hardwickii. (A–C) Morphology of highly branched cucumber hardwickii (A), moderately branched cucumber line Gy14 (B), and rarely branched line R1461 (C). lb, lateral branch. (D and E) Expression analyses of CsBRC1 by qRT-PCR in 3 cucumber lines (D) and in different organs of cucumber line R1461 (E). Values are means of 3 independent biological replicates from different plants. Error bars represent ±SD. HW, hardwickii; R, roots; S, stems; L, leaves; M, male buds; F, female buds; FR, fruits; T, shoot apex; LB, lateral buds. (F–P) In situ hybridization of CsBRC1 in cultivated cucumber R1461 (F–I) and wild ancestor hardwickii (J–P) during seedling development from 15 to 35 d after planting. K is the magnified image of the bottom axillary bud in J. sa, shoot apex; sam, shoot apical meristem; ab, axillary bud. (Scale bars represent 5 cm in A–C, 100 μm in F–I and K–O, and 200 μm in J and P.)