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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Ocul Surf. 2019 Mar 22;17(3):551–559. doi: 10.1016/j.jtos.2019.03.006

Figure 3. Induction of innate inflammatory and antiviral responses upon ZIKV infection of Pr. HCEC.

Figure 3.

Pr. HCECs were infected with ZIKV (Brazilian strain BeH823339 at MOI 5 for indicated time points. Infected and mock treated cells and subjected to qRT-PCR or western blotting. (A-C) qRT-PCR results (n=3) showing expression of PRRs (TLR3, RIG-I, and MDA5), inflammatory mediators (CCL5, and CXCL10), interferons (IFN-β1), and interferon induced genes (ISG15, OAS 2, and MX1) represented as mean ± SD, * p<0.05, ** p<0.005, *** p<0.0005, Student’s t-test. (D) Western blot analysis showing induced expression of TLR3 and ISG15 in ZIKV infected Pr. HCEC in time-dependent manner. Bar graph represents densitometry analysis of Western blots using Image J software with respect to housekeeping genes β-actin for TLR3 and HSP90 for ISG15, respectively. Mean ± SD; n=3, * p<0.05; Student’s t-test.