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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Ocul Surf. 2019 Mar 22;17(3):551–559. doi: 10.1016/j.jtos.2019.03.006

Figure 6. Infectivity and innate response of Pr. HCECs with Dengue virus.

Figure 6.

(A) Pr. HCECs were infected with DENV serotypes 1, 2, 3, and 4 at MOI 5 for 48h. Infected cells were subjected to immunostaining using 4G2 antibody, and representative images (n=3) showed the presence of DENV (green) and DAPI (blue). (B) Pr. HCECs were infected with either ZIKV Brazilian strain or DENV3 at MOI: 5 for indicated time points and qRT-PCR was performed to assess the expression of indicated PRRs (TLR3, and RIG-I), inflammatory mediators (IL-1β and TNF-α), and interferon induced genes (ISG15, and OAS2). The values have been presented as mean ± SD, * p<0.05, ** p<0.005, *** p<0.0005, Student’s t-test.