Table 1.

R-47 does not interfere with paclitaxel-induced DNA fragmentation or G2/M arrest of NSCLC cells. A549 (A) and H460 (B) cells were treated with R-47, paclitaxel, or the combination for 48 hours. Cell cycle analysis was determined by propidium iodide staining and subsequent flow cytometry analysis.

A	A549
		Phase of Cell Cycle
	48 h Treatment	% Sub-G1 (SEM)	% G0/G1 (SEM)	% S (SEM)	% G2/M (SEM)	% Polyploid (SEM)
		Control	1.0 (0.3)	54.6 (5.0)	14.9 (1.6)	26.1 (1.5)	2.3 (1.3)
		R-47 1 μM	0.9 (0.2)	61.9 (1.0)*	12.0 (0.6)	23.4 (1.0)	1.0 (0.2)
		Paclitaxel 100 nM	19.1 (1.0)†	19.4 (1.0)†	13.8 (0.8)	35.7 (1.9)$	11.2 (2.3)**
		Paclitaxel + R47	19.9 (1.7)†	23.2 (1.2)†	14.8 (0.2)	34.7 (1.7)**	6.1 (0.9)
	H460
		Phase of Cell Cycle
	48 h Treatment	% Sub-G1 (SEM)	% G0/G1 (SEM)	% S (SEM)	% G2/M (SEM)	% Polyploid (SEM)
	Control	2.7 (0.8)	59.2 (0.5)	11.0 (0.1)	25.2 (0.4)	1.8 (0.3)
	R-47 1 μM	3.0 (0.3)	58.4 (0.6)	10.8 (0.2)	25.7 (0.4)	2.1 (0.3)
B	Paclitaxel 100 nM	10.1 (0.8)†	38.9 (1.8)†	12.2 (0.2)	34.8 (0.9)†	4.0 (0.4)
	Paclitaxel + R47	9.1 (0.4)†	38.0 (1.8)†	11.3 (0.4)	37.5 (1.5)†	4.2 (0.4)

^*P < 0.05

^**P < 0.01

^$P < 0.001

^†P < 0.0001 vs control.

Data are expressed as the mean of three independent experiments.

SEM, standard error of the mean.