Table 1. Cluster analysis of HR/LR hippocampus and amygdala transcriptome data points to alterations of metabolism and mitochondrial function.
Previous gene expression microarray datasets from adult male HR/LR amygdala and hippocampus were used for DAVID cluster analysis. This analysis identified functional clusters among genes that were differentially expressed in the HR/LR brain. The top three functional cluster terms are shown along with the total number of genes in that cluster, number of genes altered between HR and LR, minimum percentage of enriched genes within one term in the cluster, and the maximum p-value of individual pathways within the cluster. Overall cluster enrichment score is listed for each cluster. The top 10 clusters are ranked from highest to lowest enrichment score.
| DAVID cluster analysis of HR/LR adult hippocampal gene expression | |||||
|---|---|---|---|---|---|
| Cluster # | Up Keyword Enrichment Term | # of altered in pathway | Minimum % of enriched genes | Cluster Enrichment Score | Maximum P-value of Pathways |
| 1 | Cell membrane, Membrane, Receptor | 174 | 17.791 | 8.468 | 2.30E-05 |
| 2 | Cell membrane, Receptor, Transducer | 83 | 13.599 | 6.109 | 2.58E-07 |
| 3 | Cell junction, Synapse, Postsynaptic cell membrane | 22 | 2.249 | 5.750 | 5.76E-06 |
| 4 | Lipoprotein, Palmitate | 29 | 2.965 | 4.765 | 1.27E-06 |
| 5 | Sodium transport, Sodium, Sodium channel | 5 | 1.534 | 3.033 | 0.039 |
| 6 | Mitochondrion, Transit peptide | 31 | 3.170 | 2.946 | 2.53E-04 |
| 7 | Glycoprotein, Disulfite bond, Secreted | 73 | 7.464 | 2.746 | 5.62E-04 |
| 8 | Ion channel, Voltage-gated channel | 16 | 1.636 | 2.306 | 9.87E-04 |
| 9 | Glucose metabolism, Carbohydrate metabolism | 5 | 0.511 | 1.900 | 9.43E-03 |
| 10 | Potassium transport, Potassium, Potassium channel | 9 | 0.920 | 1.885 | 1.34E-02 |
| DAVID cluster analysis of HR/LR adult amygdalar gene expression | |||||
| Cluster # | Up Keyword or KEGG Pathway Enrichment Term | # of altered in pathway | Minimum % of enriched genes | Cluster Enrichment Score | Maximum P-value of Pathways |
| 1 | Cell junction, synapse, postsynaptic cell membrane | 54 | 2.410 | 25.914 | 6.17E-16 |
| 2 | Mitochondrion, Mitochondrion inner membrane | 60 | 2.679 | 16.028 | 4.71E-16 |
| 3 | Mitochondrion, Transit peptide | 83 | 3.705 | 12.492 | 4.75E-14 |
| 4 | Nucleotide-binding, Kinase, ATP-binding | 139 | 6.205 | 8.776 | 3.60E-18 |
| 5 | Long-term potentiation, Circadian entrainment, Amphetamine addiction | 25 | 1.116 | 6.753 | 4.83E-06 |
| 6 | Glutamergic synapse, Circadian entrainment, Retrograde endocannabinoid signaling | 33 | 1.473 | 6.228 | 1.45E-05 |
| 7 | Ribonucleoprotein, Ribosome, Ribosomal protein | 60 | 2.679 | 6.028 | 6.65E-11 |
| 8 | GABAergic synapse, Retrograde endocannabinoid signaling, Morphine addiction | 30 | 1.339 | 5.544 | 2.67E-05 |
| 9 | Membrane, Transmembrane, Transmembrane helix | 689 | 30.759 | 5.501 | 1.19E-01 |
| 10 | Glycoprotein, Disulfide bond | 303 | 13.527 | 5.008 | 1.87E-06 |