Fig. 3.

Generation of transgenic rice plants carrying the Cas9 transgene with a sgRNA targeting the Os8N3 gene. Genotyping was performed using the specific primers for Cas9, Cas9_RT_F and Cas9_RT_R (see Fig. 2b), from four independently transformed plants and their progenies (OsU6a xa13m/Kit T₀, T₁, T₂, and T₃ generations). Genomic DNAs were extracted from Kit (Kitaake) and OsU6a xa13m/Kit T₀ (a), T₁ (b), T₂ (c), and T₃ (d). ‘ × ’ indicates PCR negative