Fig. 4.

CRISPR/Cas9-mediated mutagenesis in both Os8N3 alleles conferred enhanced resistance to Xoo. a Bacterial blight resistance phenotypes of the xa13 mutant rice lines (T₁). Rice plants 12 DAI with Xoo. From left to right: Kitaake (Kit), transgenic line (XA21, 7A-8) carrying Xa21 driven by the ubiquitin promoter, and transgenic lines (OsU6a xa13m/Kit, T₁) carrying the OsU6a::xa13m-sgRNA/pHAtC construct. Arrowheads indicated the end of the lesion. WT; wild type: Ho; homozygous: Bi; bi-allelic: He; Heterozygous. b Lesion lengths measured 12 DAI in Kitaake, XA21, and OsU6a xa13m/Kit T₁. Error bars in the graph represent standard error of at least three leaves from each plant. Letters indicate a significant difference at P < 0.050 by Tukey’s HSD test