Fig. 5.

Homozygous mutants in both Os8N3 alleles displayed enhanced resistance to Xoo. Transgenic Kitaake plants targeting xa13 (OsU6a xa13m/Kit T₂) display enhanced resistance to Xoo. a Inoculation results for mutant rice lines 12 DAI with Xoo. From left to right: Kitaake (Kit), transgenic line (XA21, 7A-8) carrying Xa21 driven by the ubiquitin promoter, and transgenic lines (OsU6a xa13m/Kit, T₂) carrying the OsU6a::xa13m-sgRNA/pHAtc construct. Arrowheads indicated the end of the lesion. He; Heterozygous; WT; wild type: Ch; chimeric: Ho; homozygous. b Lesion lengths measured 12 DAI in Kitaake, XA21, and OsU6a xa13m/Kit T₂. Error bars in the graph represent standard error of at least three leaves from each plant. Letters indicate a significant difference at P < 0.050 by Tukey’s HSD test. c Bacterial population in Kitaake, XA21, and OsU6a xa13m/Kit T₂ plants 0 and 12 DAI, determined by the number of CFU per inoculated leaf. Error bars represent standard deviation from at least three technical replicates. Letters indicate a significant difference at P < 0.050 by Tukey’s HSD test