Table 1.

Transmission and segregation of CRISPR/Cas9-mediated target mutagenesis from T₀, T₁, T₂, and T₃ of the OsU6a xa13m/Kit transgenic plant. The recovered mutated alleles of the xa13/Os8N3 gene in the OsU6a xa13m/Kit transgenic plant are shown below the Kitaake sequence. Nucleotide sequences at the target sites are shown in black capital letters and black dashes. PAM motifs are underlined. Red capital letters indicate the inserted nucleotide. The genotype of the mutation is indicated at the right of each sequence. WT indicates the nucleotide sequences identical to the Os8N3 gene in Kitaake plants. “+” indicates the insertion of the indicated number of nucleotides. No transgene: PCR negative for Cas9 gene; Transgenic: PCR positive for Cas9 gene; S: susceptible to PXO99; R: resistant to PXO99; Not available: inoculation data are not available