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. 2019 Aug 24;12:68. doi: 10.1186/s12284-019-0326-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Histochemical staining of spl40 and WT. a and d Evans blue staining: a before lesion development. d after lesion development. b and e DAB staining: b before lesion development. e after lesion development. c and f NBT staining: c before lesion development. f after lesion development. g Malonaldehyde (MDA) content at seedling and tillering stage. h Membrane ion leakage rate at seedling and tillering stage. i Expression analysis of PCD-related genes at tillering stage. Values are means ± SD (n = 3); ** indicates significance at P ≤ 0.01 and * indicates significance at P ≤ 0.05 by Student’s t test