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. Author manuscript; available in PMC: 2019 Aug 25.
Published in final edited form as: Bioconjug Chem. 2017 Jun 30;28(8):2114–2124. doi: 10.1021/acs.bioconjchem.7b00296

Figure 3.

Figure 3.

Analysis of scaled up cross-linking reaction between polyG-GFP and P22-LPETG. (A) SDS-PAGE gel evaluating the cross-linking in the polyG-GFP/P22-LPETG ligation reaction mixture after 4 h and pellet and supernatant fractions resulting from ultracentrifugation of the reaction mixture. (B) Image showing the P22 VLP pellet from ultracentrifugation after sortase mediated ligation with polyG-GFP. (C) Comparison of CsCl gradient results for P22 without GFP and resuspended P22-GFP from ultracentrifugation pellet. (D) SDS-PAGE results from the CsCl purification of the P22-GFP bands from the cesium chloride gradient of the ligation reaction in panel C.