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Evaluation of sortase mediated cross-linking of the polyG-HAhead to P22-LPETG VLP. (A) SDS-PAGE showing the characterization of individual components used in the ligation experiments. (B) Purification of P22-HAhead fusion VLPs by ultracentrifugation through a cesium chloride gradient. (C) SDS-PAGE analysis of the band corresponding to the P22 VLP from cesium chloride purification; in A and C, SP identifies the scaffolding protein of the P22 VLP.
