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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: J Invest Dermatol. 2019 Mar 13;139(9):1889–1897.e4. doi: 10.1016/j.jid.2019.02.026

Figure 5. Induction of ATF4/SESN2 pathway after Cl-amidine treatment.

Figure 5.

Reconstructed human epidermis (n = 4) were untreated (Co) or treated with 800 μM of Cl-amidine (Cl-a) for 48 h. (a) Expression of SESN2, MAP1LC3B, ATG5, and ATF4 genes was analyzed by RT-qPCR. (b) Nonidet P40 soluble proteins were immunodetected with anti-SESN2 and anti-actin antibodies (upper left). Total protein extracts were immunodetected with anti-p62/SQSTM1 and anti-actin antibodies (lower left). Molecular mass marker is indicated in kDa on the right. Immunodetected sestrin-2 and p62 were quantified, normalized to actin and expressed relatively to the control (right).