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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: J Invest Dermatol. 2019 Apr 5;139(9):2039–2042.e2. doi: 10.1016/j.jid.2019.03.1141

Figure 2. Depletion of HOPX inhibits late epidermal differentiation.

Figure 2.

(A) Western blot following short hairpin RNA-induced depletion of HOPX (shHOPX). shCTRL = control. (B) RNA-sequencing of HOPX-depleted keratinocytes. Heatmap representing the differentially-expressed genes (DEGs) associated with HOPX depletion. DEG threshold was assigned as fold change (FC) >2 or <−2, calculated false discovery rate (FDR)<0.01. (C) Gene Ontology (GO) enrichment analysis of DEGs altered by HOPX depletion using PANTHER classification. (D) Quantitative RT-PCR of differentiation-associated transcripts with HOPX depletion using two independent short hairpin RNAs against HOPX. N=3, error bars=SEM. * p<0.05. ns = not significant. (E) Immunofluorescence staining of control and HOPX-depleted regenerated epithelial tissue. (F) Model of genetic p63-ZNF750-HOPX relationship in terminal keratinocyte differentiation.