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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 May 28;192:105383. doi: 10.1016/j.jsbmb.2019.105383

FIGURE 2: Neurosteroids photolabel the ELIC-α1GABAAR subunit with a stoichiometry of two.

FIGURE 2:

(A) Structures of allopregnanolone and the photolabeling reagents, KK123 and KK200. (B) Full spectrum of intact ELIC-α1GABAAR photolabeled with 100 μM KK123 three times. (C) and (D) Deconvoluted spectra of ELIC-α1GABAAR photolabeled with KK123 or KK200 at 100 μM three times. The number of photolabeling reagents covalently modifying each protein subunit is indicated by the number above the peak.