TABLE 1:
ELIC-α1GABAAR | Intact protein MS | Middle-down MS | |
---|---|---|---|
TM3 | TM4 | ||
WT | 11.3 | 8.0 | 1.8 |
Q242L | 11.8 | 6.1 | 1.9 |
Q242W | 11.7 | 5.2 | 1.7 |
W246L | 11.5 | 3.7 | 1.2 |
Labeling efficiencies of KK200 (%) in intact protein MS and middle-down MS analyses for the ELIC-α1GABAAR WT and the mutated receptors.