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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Kidney Int. 2019 May 6;96(3):656–673. doi: 10.1016/j.kint.2019.03.023

Figure 1: MB is induced during recovery of podocytes from injury:

Figure 1:

(A) Podocytes treated with PS showed actin cytoskeleton (Green) disorganization with accumulation of actin stress fibers at the cell periphery, and reduced Neph1 (Red) at cell-cell junctions. Recovery induced with supplementation of serum (serum induced recovery) restored actin cytoskeleton organization and localization of Neph1 at the cell junctions. Scale bar 20μm. (B) The quantitative analysis showed significant increase in the number of healthy podocytes (>40%) during recovery. (C) The quantitative assessment of Neph1 localization showed significant relocalization of Neph1 (~40%) at the cell membrane during recovery. (D) Analysis of mtDNA copy number showed significant enhancement during recovery of podocytes from injuries with NTS or PS. (E) Upregulation of β2-AR2 and various other mitochondrial components as evaluated by qPCR. All experiments were performed at least in triplicates. Data are presented in mean±SEM and p-values were calculated using a 2-tailed t-test. *P≤0.05, **P≤0.01, control vs. injury; aP≤0.05, PS vs. PS+recovery; bP≤0.05 NTS vs. NTS+recovery.