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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Kidney Int. 2019 May 6;96(3):656–673. doi: 10.1016/j.kint.2019.03.023

Figure 7: Formoterol treatment restored Neph1 localization at the podocyte cell membrane and reduced cellular apoptosis:

Figure 7:

(A) Mice kidney sections were immunostained with Neph1 (Green) and Synaptopodin (Red) antibodies and DAPI (Blue). NTS induced mislocalization of Neph1 was largely restored by formoterol treatment, where increased Neph1 localization at the podocytes membrane and colocalization with synaptopodin was visible. (B) The Pearson’s correlation coefficient (Rr) analysis showed increased colocalization of Neph1 and Synaptopodin in NTS+formoterol treated mice. Data are presented in mean±SEM. One-way ANOVA, ***P≤0.001 NTS+vehicle vs. NTS+formoterol. (C & D) Apoptosis was measured in the kidney sections using TUNEL assay. Significant amounts of TUNEL positive (Green) nuclei (blue DAPI) were present in NTS+vehicle treated control mice and positive control, whereas they were largely absent in the NTS+formoterol treated mice (white arrows). Data are presented in mean±SEM. One-way ANOVA, ###P≤0.001 Control vs NTS+vehicle; ***P≤0.001 NTS+vehicle vs. NTS+formoterol.